Comprehensive Molecular Bioengineering Lab
This laboratory is equipped to support molecular biology, fermentation, and analytical research.
CONTACT: Dr. Mark Eiteman
Molecular biological facilities include:
- microplate readers
- dual-label scintillation counter
- freeze drier
- DNA gel electrophoresis (4)
- SDS-PAGE with western blot capability (4)
- DNA imaging system
- speed-vac
- fast ramp thermocycler (2)
- electroporator
- UV crosslinker
- chromatography refrigerator
- dishwasher
- autoclave
- drying oven
- cold room
- automatic Petri-plate pouring machine
- microfuges (4)
- freezers (3)
Fermentation facilities include:
- 2.5 liter fermenters (9)
- 5.0 liter fermenter
- 19 liter fermenter, incubators (8)
- biosafety cabinets (3)
- French pressure cell
- centrifuges (3)
- crushed ice makers (2)
- anaerobic glove box, and water bath/shakers (7)
Temperature, pH, and oxygen, carbon dioxide off-gas monitoring and control are used. Online glucose, lactate, glutamine and glutamate with feed-pump control are available.
Chemical analysis facilities include:
- Gas chromatographs (3)
- Spectrophotometers (3) and liquid chromatographs (4) with fluorometric, refractive index, UV/visible, and conductivity detectors
- Portable GC/MS instruments (2) are available for gas or headspace analysis
- Dual column GC/MS with thermodesorption, cryofocussing and sniff port